The Rat GMFB (Glia Maturation Factor Beta) CLIA Kit is a highly sensitive and specific assay designed for the accurate measurement of GMFB levels in rat samples. This kit is ideal for researchers studying the role of GMFB in neural development, synaptic plasticity, and neurodegenerative diseases.GMFB is a key protein in the central nervous system, playing a crucial role in regulating neuronal growth and function.
Dysregulation of GMFB has been linked to various neurological disorders, making it a valuable biomarker for understanding and potentially treating these conditions.With its reliable performance and ease of use, the Rat GMFB CLIA Kit is an essential tool for investigating the function and significance of GMFB in both basic research and clinical studies.
Product Name:
Rat GMFB (Glia Maturation Factor, Beta) CLIA Kit
SKU:
RTES00237
Target:
Rat GMFB (Glia Maturation Factor, Beta)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
1.88 ng/mL
Detection range:
3.13-200 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat GMFB. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat GMFB and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat GMFB, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat GMFB. You can calculate the concentration of Rat GMFB in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-99
95-113
91-102
Average (%)
94
103
96
1:4
Range (%)
84-98
85-95
98-111
Average (%)
91
90
106
1:8
Range (%)
98-116
85-97
98-115
Average (%)
107
91
105
1:16
Range (%)
95-110
94-106
100-116
Average (%)
100
100
106
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-102
93
EDTA plasma (n=5)
86-99
93
Cell culture media (n=5)
88-104
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
10.22
30.84
73.77
11.14
28.25
70.12
Standard deviation
1.03
3.66
6.08
1.32
2.02
6.84
C V (%)
10.08
11.87
8.24
11.85
7.15
9.75
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat GMFB concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat GMFB in samples. No significant cross-reactivity or interference between Rat GMFB and analogues was observed.
