The Rat GNB1 (G Protein Beta-1) CLIA Kit is a powerful tool for the quantitative detection of G Protein Beta-1 levels in rat samples including serum, plasma, and tissue homogenates. This kit offers exceptional sensitivity, precision, and accuracy, ensuring reliable and consistent results for your research needs.G Protein Beta-1 is a key component of G protein signaling pathways, playing a crucial role in cellular signal transduction.
Dysregulation of G protein signaling has been implicated in various diseases, making G Protein Beta-1 a valuable biomarker for studying pathophysiological mechanisms and potential therapeutic targets.With the Rat GNB1 CLIA Kit, researchers can confidently measure G Protein Beta-1 levels in rat samples, advancing their understanding of G protein signaling and its implications in disease development and progression.
Product Name:
Rat GNb1 (G Protein Beta 1) CLIA Kit
SKU:
RTES00218
Target:
Rat GNb1 (G Protein Beta 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
1.88 μIU/mL
Detection range:
3.13-200 μIU/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat GNb1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat GNb1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat GNb1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat GNb1. You can calculate the concentration of Rat GNb1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-106
86-97
97-113
Average (%)
100
92
103
1:4
Range (%)
84-96
93-106
91-102
Average (%)
90
101
97
1:8
Range (%)
101-112
103-117
87-103
Average (%)
106
109
95
1:16
Range (%)
92-107
94-106
101-114
Average (%)
99
99
107
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-100
93
EDTA plasma (n=5)
88-102
94
Cell culture media (n=5)
101-113
106
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
9.47
22.56
83.48
8.98
24.35
82.36
Standard deviation
1.06
1.92
7.71
1.15
1.77
8.24
C V (%)
11.19
8.51
9.24
12.81
7.27
10.0
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat GNb1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat GNb1 in samples. No significant cross-reactivity or interference between Rat GNb1 and analogues was observed.
