Rat GnRHR (Gonadotropin-Releasing Hormone Receptor) CLIA Kit
The Rat GNRHR (Gonadotropin-Releasing Hormone Receptor) CLIA Kit is a reliable and accurate tool for measuring GNRHR levels in rat samples including serum, plasma, and tissue lysates. This kit offers high sensitivity and specificity, ensuring precise and consistent results for a variety of research purposes.Gonadotropin-Releasing Hormone Receptor is a key player in the regulation of reproductive functions in mammals, influencing the release of hormones that control fertility. Abnormalities in GNRHR signaling have been linked to conditions such as infertility, hormonal imbalances, and reproductive disorders.
By measuring GNRHR levels, researchers can gain valuable insights into the mechanisms underlying these conditions and potentially identify therapeutic targets.Overall, the Rat GNRHR CLIA Kit is an essential tool for studying reproductive physiology and developing interventions for reproductive health conditions in rats. Its accuracy and reliability make it a valuable asset for researchers in the fields of endocrinology, reproductive biology, and drug development.
Product Name:
Rat GnRHR (Gonadotropin-Releasing Hormone Receptor) CLIA Kit
SKU:
RTES00258
Target:
Rat GnRHR (Gonadotropin-Releasing Hormone Receptor)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat GnRHR. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat GnRHR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat GnRHR, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat GnRHR. You can calculate the concentration of Rat GnRHR in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
102-116
88-101
91-103
Average (%)
110
95
96
1:4
Range (%)
91-104
88-103
90-106
Average (%)
97
94
97
1:8
Range (%)
100-114
92-106
90-101
Average (%)
107
97
96
1:16
Range (%)
87-98
99-111
90-104
Average (%)
93
105
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-109
100
EDTA plasma (n=5)
97-111
104
Cell culture media (n=5)
89-100
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
201.41
608.07
1511.96
187.46
663.09
1362.3
Standard deviation
23.46
58.8
151.35
19.93
55.9
102.04
C V (%)
11.65
9.67
10.01
10.63
8.43
7.49
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat GnRHR concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat GnRHR in samples. No significant cross-reactivity or interference between Rat GnRHR and analogues was observed.
