Rat GP4/CD36 (Platelet Membrane Glycoprotein IV) CLIA Kit
The Rat GP4 (CD36) Platelet Membrane Glycoprotein IV CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of GP4 levels in rat samples. This kit is ideal for studying the role of GP4 in platelet function and thrombosis, as well as its potential involvement in various diseases and disorders.Platelet membrane glycoprotein IV (GP4), also known as CD36, is a key player in platelet adhesion, activation, and aggregation processes.
Its dysregulation has been associated with thrombotic disorders, atherosclerosis, and other cardiovascular diseases, highlighting its importance as a potential therapeutic target.With its reliable and reproducible results, the Rat GP4 (CD36) Platelet Membrane Glycoprotein IV CLIA Kit is an essential tool for researchers investigating the function and regulation of GP4 in both normal physiological processes and pathological conditions.
Product Name:
Rat GP4/CD36 (Platelet Membrane Glycoprotein IV) CLIA Kit
SKU:
RTES00567
Target:
Rat GP4/CD36 (Platelet Membrane Glycoprotein IV)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat GP4/CD36. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat GP4/CD36 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat GP4/CD36, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat GP4/CD36. You can calculate the concentration of Rat GP4/CD36 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-115
91-104
90-105
Average (%)
107
96
96
1:4
Range (%)
87-102
103-117
96-111
Average (%)
94
108
103
1:8
Range (%)
97-111
97-114
103-116
Average (%)
105
104
109
1:16
Range (%)
96-114
92-108
94-109
Average (%)
104
99
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
84-98
91
EDTA plasma (n=5)
86-97
92
Cell culture media (n=5)
98-111
105
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
211.27
539.24
1892.86
226.02
525.19
2064.03
Standard deviation
25.94
46.37
127.2
22.53
42.12
159.14
C V (%)
12.28
8.6
6.72
9.97
8.02
7.71
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat GP4/CD36 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat GP4/CD36 in samples. No significant cross-reactivity or interference between Rat GP4/CD36 and analogues was observed.
