Rat GR alpha (Glucocorticoid Receptor alpha) CLIA Kit
The Rat GR Alpha (Glucocorticoid Receptor Alpha) CLIA Kit is specifically designed for the quantitative measurement of GR Alpha levels in rat samples. This kit offers high sensitivity and specificity, allowing for accurate and reliable results in serum, plasma, and cell culture supernatants.GR Alpha is a key protein involved in the regulation of gene expression in response to glucocorticoid hormones.
Dysregulation of GR Alpha has been implicated in various diseases such as inflammatory disorders, metabolic syndrome, and stress-related conditions. Therefore, this kit is an essential tool for researchers studying the role of GR Alpha in these diseases and for developing potential therapeutic interventions.
Product Name:
Rat GR alpha (Glucocorticoid Receptor alpha) CLIA Kit
SKU:
RTES00240
Target:
Rat GR alpha (Glucocorticoid Receptor alpha)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat GRα. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat GRα and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat GRα, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat GRα. You can calculate the concentration of Rat GRα in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-98
87-99
90-103
Average (%)
93
92
98
1:4
Range (%)
88-104
88-99
86-101
Average (%)
95
93
93
1:8
Range (%)
84-96
93-107
96-106
Average (%)
91
100
101
1:16
Range (%)
94-106
85-98
100-118
Average (%)
100
91
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-115
107
EDTA plasma (n=5)
93-110
101
Cell culture media (n=5)
92-108
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
218.92
571.5
1369.83
206.6
560.27
1489.79
Standard deviation
23.93
40.29
139.31
20.12
65.78
158.66
C V (%)
10.93
7.05
10.17
9.74
11.74
10.65
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat GRα concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat GRα in samples. No significant cross-reactivity or interference between Rat GRα and analogues was observed.
