Description
Rat GRbeta (Glucocorticoid Receptor Beta) ELISA Kit
The Rat GR? (Glucocorticoid Receptor Beta) ELISA Kit is a highly specialized assay designed to quantitatively measure glucocorticoid receptor beta levels in various rat biological samples. Glucocorticoid receptor beta is a specific isoform of the glucocorticoid receptor that plays a critical role in modulating cellular responses to glucocorticoid hormones.
As a key regulator of gene transcription, GR? is involved in diverse physiological processes such as metabolism, immune function, and stress responses. Its unique properties and functions make it an important target for research into endocrine signaling pathways and associated cellular mechanisms. This ELISA kit provides a reliable and accurate method for assessing and analyzing GR? levels, offering valuable insights into the intricate roles of this isoform in gene regulation, stress signaling, and immune modulation. Accurate measurement of GR? is essential for investigating its contribution to various biological pathways and its potential implications in disease states and pharmacological interventions. The Rat GR? ELISA Kit from Assay Genie offers exceptional sensitivity and specificity, ensuring precise and reproducible results. Manufactured under strict quality control standards, this kit delivers robust performance and ease of use, making it an excellent choice for researchers studying the functions and significance of glucocorticoid receptor beta in biological processes.
| Product Name: | Rat GRbeta (Glucocorticoid Receptor Beta) ELISA Kit |
| Product Code: | AEES00410 |
| Assay Type: | Sandwich |
| Format: | 96T |
| Assay Time: | 3.5h |
| Reactivity: | Rat |
| Detection Range: | 15.63-1000 pg/mL |
| Sensitivity: | 9.38 pg/mL |
| Sample Type & Sample Volume: | Serum, plasma and other biological fluids, 100?L |
| Specificity: | This kit recognizes Rat GR? in samples. No significant cross-reactivity or interference between Rat GR? and analogues was observed. |
| Reproducibility: | Both intra-CV and inter-CV are < 10%. |
| Application: | This ELISA kit applies to the in vitro quantitative determination of Rat GR? concentrations in Serum, plasma and other biological fluids. |
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat GR?. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat GR? and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat GR?, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat GR?. You can calculate the concentration of Rat GR? in the samples by comparing the OD of the samples to the standard curve.
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat GR? were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat GR? were tested on 3 different plates, 20 replicates in each plate.
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 45.49 | 111.44 | 495.11 | 43.50 | 114.85 | 539.40 |
| Standard deviation | 2.48 | 4.59 | 15.00 | 3.04 | 6.86 | 24.27 |
| CV (%) | 5.45 | 4.12 | 3.03 | 6.99 | 5.97 | 4.50 |
Recovery
The recovery of Rat GR? spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 89-99 | 94 |
| EDTA plasma (n=8) | 91-104 | 98 |
| Cell culture media (n=8) | 90-101 | 95 |
| Kit Components: | An unopened kit can be stored at 2-8? for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
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| 1. | Add 100?L standard or sample to the wells. Incubate for 90 min at 37°C |
| 2. | Discard the liquid, immediately add 100?L Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C |
| 3. | Aspirate and wash the plate for 3 times |
| 4. | Add 100?L HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times |
| 5. | Add 90?L Substrate Reagent. Incubate for 15 min at 37°C |
| 6. | Add 50?L Stop Solution |
| 7. | Read the plate at 450nm immediately. Calculation of the results |
