Rat GRB10 (Growth Factor Receptor Bound Protein 10) CLIA Kit
The Rat GRB10 (Growth Factor Receptor-Bound Protein 10) CLIA Kit is a reliable and sensitive assay designed for the accurate quantification of GRB10 levels in rat serum, plasma, and cell culture supernatants. This kit provides high specificity and reproducibility, making it a valuable tool for researchers in various fields.GRB10 is a key protein that plays a crucial role in signaling pathways involved in cell growth, differentiation, and metabolism. Dysregulation of GRB10 has been linked to various diseases, including cancer, diabetes, and obesity, making it a promising target for therapeutic interventions.
By accurately measuring GRB10 levels, researchers can gain insights into the role of this protein in disease progression and potentially identify novel treatment strategies. The Rat GRB10 CLIA Kit is an essential tool for studying the functional significance of GRB10 and exploring its potential as a diagnostic or therapeutic target.
Product Name:
Rat GRB10 (Growth Factor Receptor Bound Protein 10) CLIA Kit
SKU:
RTES00270
Target:
Rat GRB10 (Growth Factor Receptor Bound Protein 10)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat GRB10. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat GRB10 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat GRB10, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat GRB10. You can calculate the concentration of Rat GRB10 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-113
96-110
93-110
Average (%)
104
103
100
1:4
Range (%)
100-118
89-100
96-112
Average (%)
108
95
102
1:8
Range (%)
88-102
94-111
92-104
Average (%)
94
102
98
1:16
Range (%)
92-106
91-104
84-98
Average (%)
99
98
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
98-111
104
EDTA plasma (n=5)
92-105
99
Cell culture media (n=5)
93-107
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
96.02
297.65
717.77
89.02
273.79
746.53
Standard deviation
8.97
31.22
73.86
9.33
23.22
74.35
C V (%)
9.34
10.49
10.29
10.48
8.48
9.96
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat GRB10 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat GRB10 in samples. No significant cross-reactivity or interference between Rat GRB10 and analogues was observed.
