Rat GRB14 (Growth Factor Receptor Bound Protein 14) CLIA Kit
The Rat GRB14 (Growth Factor Receptor Bound Protein 14) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of GRB14 levels in rat samples. This kit is ideal for researchers studying signal transduction pathways and the role of GRB14 in various cellular processes.GRB14 is a key adaptor protein that interacts with growth factor receptors and plays a critical role in regulating cell proliferation, differentiation, and survival.
Dysregulation of GRB14 is associated with a variety of diseases, including cancer, diabetes, and obesity, making it an important target for therapeutic intervention.With its high sensitivity and specificity, the Rat GRB14 CLIA Kit provides reliable and reproducible results, making it a valuable tool for studying the biology of GRB14 and its potential implications in disease pathology.
Product Name:
Rat GRB14 (Growth Factor Receptor Bound Protein 14) CLIA Kit
SKU:
RTES00271
Target:
Rat GRB14 (Growth Factor Receptor Bound Protein 14)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat GRB14. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat GRB14 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat GRB14, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat GRB14. You can calculate the concentration of Rat GRB14 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-109
101-120
100-114
Average (%)
104
109
108
1:4
Range (%)
99-115
96-111
90-103
Average (%)
105
103
95
1:8
Range (%)
100-117
84-98
90-103
Average (%)
109
91
96
1:16
Range (%)
94-104
98-114
104-119
Average (%)
99
105
110
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-104
95
EDTA plasma (n=5)
96-112
102
Cell culture media (n=5)
86-99
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
106.56
265.8
967.63
100.6
240.13
990.77
Standard deviation
9.14
21.42
89.51
11.96
18.03
84.12
C V (%)
8.58
8.06
9.25
11.89
7.51
8.49
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat GRB14 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat GRB14 in samples. No significant cross-reactivity or interference between Rat GRB14 and analogues was observed.
