The Rat GRN (Granulin) CLIA Kit is a powerful tool for quantifying levels of granulin in rat samples such as serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit provides researchers with reliable and consistent results, making it indispensable for various research studies.Granulin is a key protein that regulates cell growth, differentiation, and survival, playing a critical role in various physiological processes. Dysregulation of granulin expression has been linked to several diseases, including neurodegenerative disorders, inflammatory conditions, and cancer, making it a valuable biomarker for studying these diseases and exploring potential therapeutic interventions.
In summary, the Rat GRN (Granulin) CLIA Kit offers researchers a precise and efficient method for analyzing granulin levels in rat samples, facilitating valuable insights into the role of granulin in health and disease.
Product Name:
Rat GRN (Granulin) CLIA Kit
SKU:
RTES00259
Target:
Rat GRN (Granulin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat GRN. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat GRN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat GRN, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat GRN. You can calculate the concentration of Rat GRN in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-106
99-113
83-96
Average (%)
100
106
90
1:4
Range (%)
97-110
91-102
98-114
Average (%)
104
97
105
1:8
Range (%)
93-104
84-100
94-108
Average (%)
99
91
100
1:16
Range (%)
88-99
90-105
85-98
Average (%)
93
97
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-103
97
EDTA plasma (n=5)
98-112
104
Cell culture media (n=5)
87-101
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
191.54
562.31
1759.87
203.4
509.31
1689.39
Standard deviation
17.87
44.93
141.67
25.67
59.79
106.77
C V (%)
9.33
7.99
8.05
12.62
11.74
6.32
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat GRN concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat GRN in samples. No significant cross-reactivity or interference between Rat GRN and analogues was observed.
