Description
Rat GRN (Granulin) ELISA Kit
The Rat GRN (Granulin) ELISA Kit is a specialized assay designed for the quantitative measurement of granulin levels in various biological samples from rats. Granulin, also known as progranulin, is a multifunctional growth factor that plays critical roles in various physiological processes, including cell growth, inflammation, and wound healing. It is particularly recognized for its involvement in neurobiology, where it influences neuronal survival, neurite outgrowth, and neuroinflammation. This ELISA kit provides a reliable and sensitive tool for researchers to assess granulin levels, offering valuable insights into its functions in health and disease.
By accurately quantifying granulin concentrations, scientists can deepen their understanding of the molecular mechanisms underlying neurodegenerative diseases, cancer progression, and immune responses. The Rat GRN (Granulin) ELISA Kit from Assay Genie ensures exceptional sensitivity and specificity, leading to reliable and reproducible results. Manufactured under stringent quality control standards, this kit delivers robust performance and ease of use, making it an excellent choice for research in neurobiology, inflammation, and other fields related to granulin biology in rat models.
| Product Name: | Rat GRN (Granulin) ELISA Kit |
| Product Code: | AEES00417 |
| Assay Type: | Sandwich |
| Format: | 96T |
| Assay Time: | 3.5h |
| Reactivity: | Rat |
| Detection Range: | 0.31-20 ng/mL |
| Sensitivity: | 0.19 ng/mL |
| Sample Type & Sample Volume: | Serum, plasma and other biological fluids, 100?L |
| Specificity: | This kit recognizes Rat GRN in samples. No significant cross-reactivity or interference between Rat GRN and analogues was observed. |
| Reproducibility: | Both intra-CV and inter-CV are < 10%. |
| Application: | This ELISA kit applies to the in vitro quantitative determination of Rat GRN concentrations in Serum, plasma and other biological fluids. |
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat GRN. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat GRN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat GRN, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat GRN. You can calculate the concentration of Rat GRN in the samples by comparing the OD of the samples to the standard curve.
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat GRN were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat GRN were tested on 3 different plates, 20 replicates in each plate.
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) | 1.02 | 2.70 | 7.66 | 0.94 | 2.96 | 7.37 |
| Standard deviation | 0.06 | 0.12 | 0.24 | 0.05 | 0.16 | 0.37 |
| CV (%) | 5.88 | 4.44 | 3.13 | 5.32 | 5.41 | 5.02 |
Recovery
The recovery of Rat GRN spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 93-107 | 98 |
| EDTA plasma (n=8) | 87-101 | 94 |
| Cell culture media (n=8) | 88-104 | 95 |
| Kit Components: | An unopened kit can be stored at 2-8? for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
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| 1. | Add 100?L standard or sample to the wells. Incubate for 90 min at 37°C |
| 2. | Discard the liquid, immediately add 100?L Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C |
| 3. | Aspirate and wash the plate for 3 times |
| 4. | Add 100?L HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times |
| 5. | Add 90?L Substrate Reagent. Incubate for 15 min at 37°C |
| 6. | Add 50?L Stop Solution |
| 7. | Read the plate at 450nm immediately. Calculation of the results |
