The Rat GS (Gelsolin) CLIA Kit is a highly reliable and sensitive assay designed for the accurate quantification of gelsolin levels in rat samples, including serum, plasma, and cell culture supernatants. This kit provides precise and reproducible results, making it perfect for various research applications.Gelsolin is an essential protein that plays a crucial role in actin filament severing and capping, as well as in cell motility and apoptosis. Abnormal levels of gelsolin have been linked to various diseases such as Alzheimer's, Parkinson's, and cancer.
Therefore, this kit is an invaluable tool for studying these conditions and exploring potential therapeutic interventions.With its high sensitivity and specificity, the Rat GS (Gelsolin) CLIA Kit from Assay Genie offers researchers a reliable and accurate method for measuring gelsolin levels in rat samples, contributing to advancements in biomedical research and drug development.
Product Name:
Rat GS (Gelsolin) CLIA Kit
SKU:
RTES00235
Target:
Rat GS (Gelsolin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
1.88 ng/mL
Detection range:
3.13-200 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat GS. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat GS and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat GS, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat GS. You can calculate the concentration of Rat GS in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-112
95-107
92-106
Average (%)
104
101
98
1:4
Range (%)
100-116
85-97
98-113
Average (%)
107
91
104
1:8
Range (%)
102-118
100-117
96-109
Average (%)
109
108
102
1:16
Range (%)
99-111
99-113
90-105
Average (%)
105
107
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-106
97
EDTA plasma (n=5)
89-101
95
Cell culture media (n=5)
93-105
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
9.72
23.08
76.59
9.26
22.86
72.33
Standard deviation
1.26
1.7
6.41
1.1
2.71
6.94
C V (%)
12.96
7.37
8.37
11.88
11.85
9.59
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat GS concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat GS in samples. No significant cross-reactivity or interference between Rat GS and analogues was observed.
