The Rat GZMs-B (Granzymes B) CLIA Kit is specifically designed for the accurate measurement of granzyme B levels in rat samples, including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures reliable and reproducible results, making it an ideal tool for various research applications.Granzyme B is a key component of the immune system, playing a crucial role in immune responses and cell-mediated cytotoxicity. Elevated levels of granzyme B have been associated with various inflammatory and autoimmune diseases, making it a valuable biomarker for studying these conditions and potential therapeutic interventions.
Overall, the Rat GZMs-B CLIA Kit provides researchers with a reliable and efficient method for quantifying granzyme B levels in rat samples, offering valuable insights into the immune response and the development of targeted treatments for a range of diseases.
Product Name:
Rat Gzms-B ( Granzymes B) CLIA Kit
SKU:
RTES00262
Target:
Rat Gzms-B ( Granzymes B)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat GzmB. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat GzmB and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat GzmB, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat GzmB. You can calculate the concentration of Rat GzmB in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-108
87-98
100-114
Average (%)
102
92
107
1:4
Range (%)
102-119
98-112
96-110
Average (%)
109
105
101
1:8
Range (%)
91-106
91-104
96-107
Average (%)
97
98
101
1:16
Range (%)
95-107
98-113
94-108
Average (%)
102
106
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
98-110
104
EDTA plasma (n=5)
95-107
101
Cell culture media (n=5)
89-104
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
23.04
76.55
245.14
24.69
76.85
261.61
Standard deviation
2.86
7.49
25.94
3.04
6.41
16.19
C V (%)
12.41
9.78
10.58
12.31
8.34
6.19
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat GzmB concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat GzmB in samples. No significant cross-reactivity or interference between Rat GzmB and analogues was observed.
