The Rat Haptoglobin (HPT) ELISA Kit is specifically designed for the precise measurement of haptoglobin levels in rat serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit guarantees accurate and consistent results, making it ideal for a variety of research purposes.Haptoglobin is a key protein involved in the acute phase response, playing a crucial role in binding free hemoglobin and preventing oxidative damage.
It is implicated in various inflammatory conditions, making it a valuable biomarker for studying inflammation-related diseases such as infections, autoimmune disorders, and cardiovascular diseases.Overall, the Rat Haptoglobin (HPT) ELISA Kit is a reliable tool for researchers looking to study haptoglobin levels in rat samples and investigate its role in disease pathogenesis and therapeutic interventions.
Product Name:
Rat Haptoglobin (Hpt) ELISA Kit
Product Code:
RTDL00494
Size:
96 Assays
Target:
Hpt
Synonyms:
HP, Hp2-Alpha, Alpha-2-Macroglobulin, Zonulin
Detection Method:
Sandwich
Range:
15.625-1,000ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
89-101
95
EDTA plasma (n=5)
91-100
95
Heparin plasma (n=5)
91-101
96
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
