Rat Heat Shock Protein 90kDa Alpha A1 (HSP90aA1) ELISA Kit
The Rat Heat Shock Protein 90kDa Alpha A1 (HSP90AA1) ELISA Kit is a powerful tool for accurately measuring levels of HSP90AA1 in rat serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides reliable and reproducible results, making it ideal for a variety of research applications.HSP90AA1 is a key player in the heat shock response, assisting in protein folding and stabilization under stressful conditions. It plays a vital role in various cellular processes and is implicated in numerous diseases, including cancer and neurodegenerative disorders.
Studying HSP90AA1 levels can provide valuable insights into these conditions and aid in the development of potential therapeutic strategies.This ELISA kit offers a convenient and efficient way to analyze HSP90AA1 levels in rats, enabling researchers to further their understanding of the protein's function and implications in various diseases. With its user-friendly protocol and accurate results, the Rat Heat Shock Protein 90kDa Alpha A1 ELISA Kit is a valuable asset for any laboratory conducting research in this area.
Product Name:
Rat Heat Shock Protein 90kDa Alpha A1 (HSP90aA1) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-101
91
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
