Rat Heat Shock Protein 90kDa Beta 1 (HSP90b1) ELISA Kit
The Rat Heat Shock Protein 90kDa Beta-1 (Hsp90b1) ELISA kit from Assay Genie is a reliable and accurate tool for detecting levels of Hsp90b1 in rat serum, plasma, and cell culture supernatants. With high sensitivity and specificity, this kit provides reproducible results for a variety of research applications.Hsp90b1 is a key molecular chaperone involved in protein folding and cellular stress response. Its dysregulation has been linked to various diseases including cancer, neurodegenerative disorders, and cardiovascular diseases.
Therefore, measuring Hsp90b1 levels can provide valuable insights into disease progression and potential therapeutic targets.Trust Assay Genie's Rat Heat Shock Protein 90kDa Beta-1 ELISA kit for precise quantification of Hsp90b1 levels in rat samples, aiding in your research endeavors and furthering our understanding of cellular stress responses.
Product Name:
Rat Heat Shock Protein 90kDa Beta 1 (HSP90b1) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-99
91
EDTA plasma (n=5)
80-100
90
Heparin plasma (n=5)
82-99
90
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
