The Rat Heat Shock Protein Beta-2 (HSPB2) ELISA Kit is specifically designed for the precise measurement of HSPB2 levels in rat samples, including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research applications.HSPB2 is a key protein in the heat shock response, playing a critical role in protecting cells from stress-induced damage. It is involved in maintaining cellular homeostasis and preventing protein aggregation under challenging conditions.
By studying HSPB2 levels, researchers can gain valuable insights into stress response mechanisms and potential therapeutic targets for various diseases.Overall, the Rat Heat Shock Protein Beta-2 ELISA Kit is a valuable tool for investigating the role of HSPB2 in cellular stress response pathways and exploring its implications in disease pathology. Its reliability and precision make it an essential resource for researchers dedicated to advancing our understanding of cellular stress biology.
Product Name:
Rat Heat Shock Protein Beta 2 (HSPb2) ELISA Kit
Product Code:
RTDL00510
Size:
96 Assays
Target:
HSPb2
Synonyms:
HSP-B2, MKBP, HSP27, Heat Shock 27kDa Protein 2, DMPK-binding protein
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-99
91
EDTA plasma (n=5)
80-99
89
Heparin plasma (n=5)
82-99
90
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
