Description
Rat HGF (Hepatocyte Growth Factor) ELISA Kit
The Rat HGF (Hepatocyte Growth Factor) ELISA Kit is meticulously crafted to facilitate the quantitative assessment of Hepatocyte Growth Factor levels in diverse rat biological samples.
HGF is a pivotal protein known for its critical functions in regulating cell growth, proliferation, migration, and tissue repair processes. By precisely measuring HGF concentrations, researchers gain valuable insights into its role in liver regeneration, cell migration, tissue development, and responses to injury or disease. Accurate quantification of Hepatocyte Growth Factor is paramount for unraveling its involvement in physiological processes and pathological conditions, offering potential avenues for therapeutic interventions and targeted treatments. The Rat HGF ELISA Kit boasts exceptional sensitivity and specificity, ensuring dependable and reproducible results. Crafted with rigorous quality control standards, this kit provides robust performance and user-friendly workflow, making it an indispensable tool for cutting-edge research endeavors and investigations into the multifaceted functions of HGF in biological systems.
| Product Name: | Rat HGF (Hepatocyte Growth Factor) ELISA Kit |
| Product Code: | AEES00430 |
| Assay Type: | Sandwich |
| Format: | 96T |
| Assay Time: | 3.5h |
| Reactivity: | Rat |
| Detection Range: | 62.50-4000 pg/mL |
| Sensitivity: | 37.50 pg/mL |
| Sample Type & Sample Volume: | Serum, plasma and other biological fluids, 100?L |
| Specificity: | This kit recognizes Rat HGF in samples. No significant cross-reactivity or interference between Rat HGF and analogues was observed. |
| Reproducibility: | Both intra-CV and inter-CV are < 10%. |
| Application: | This ELISA kit applies to the in vitro quantitative determination of Rat HGF concentrations in serum, plasma and other biological fluids. |
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat HGF. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat HGF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat HGF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat HGF. You can calculate the concentration of Rat HGF in the samples by comparing the OD of the samples to the standard curve.
| Kit Components: | An unopened kit can be stored at 2-8? for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
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| 1. | Add 100?L standard or sample to the wells. Incubate for 90 min at 37°C |
| 2. | Discard the liquid, immediately add 100?L Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C |
| 3. | Aspirate and wash the plate for 3 times |
| 4. | Add 100?L HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times |
| 5. | Add 90?L Substrate Reagent. Incubate for 15 min at 37°C |
| 6. | Add 50?L Stop Solution |
| 7. | Read the plate at 450nm immediately. Calculation of the results |
