Rat HLA-B Associated Transcript 3 (BAT3) ELISA Kit
The Rat BAT3 (HLA-B associated transcript 3) ELISA Kit is specifically designed for the accurate measurement of BAT3 levels in rat serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit enables researchers to obtain reliable and reproducible results for various research applications.BAT3, also known as BAG6, is a critical protein involved in regulating protein quality control and cell survival. Its dysregulation has been linked to various diseases, including cancer, autoimmune disorders, and neurodegenerative conditions.
Therefore, measuring BAT3 levels can provide valuable insights into disease mechanisms and potential therapeutic strategies.This ELISA kit provides a simple, efficient, and cost-effective solution for studying BAT3 in rat models, allowing researchers to further understand its role in physiology and pathology. Upgrade your research with the Rat BAT3 ELISA Kit today.
Product Name:
Rat HLA-B Associated Transcript 3 (BAT3) ELISA Kit
Product Code:
RTDL00131
Size:
96 Assays
Target:
BAT3
Synonyms:
BAG6, Protein G3, Protein Scythe, BCL2-associated athanogene 6, BAG family molecular chaperone regulator 6, Large proline-rich protein BAG6
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
