Rat HNF1a (Hepatocyte Nuclear Factor 1 Alpha) CLIA Kit
The Rat HNF1A (Hepatocyte Nuclear Factor 1 Alpha) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of HNF1A levels in rat samples including serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it an essential tool for research focusing on liver function, metabolism, and gene regulation.HNF1A is a key transcription factor involved in regulating genes essential for liver development, glucose metabolism, and lipid homeostasis.
Dysregulation of HNF1A has been linked to various liver disorders, diabetes, and metabolic diseases, highlighting its significance as a potential therapeutic target.With its high performance and versatility, the Rat HNF1A CLIA Kit is an indispensable tool for researchers investigating liver biology, metabolic diseases, and gene expression regulation in rats. Get accurate and reliable results with this advanced assay kit from Assay Genie.
Product Name:
Rat HNF1a (Hepatocyte Nuclear Factor 1 Alpha) CLIA Kit
SKU:
RTES00293
Target:
Rat HNF1a (Hepatocyte Nuclear Factor 1 Alpha)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat HNF1a. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat HNF1a and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat HNF1a, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat HNF1a. You can calculate the concentration of Rat HNF1a in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-108
100-118
92-105
Average (%)
102
108
99
1:4
Range (%)
87-103
95-108
88-104
Average (%)
94
100
95
1:8
Range (%)
95-105
97-114
93-109
Average (%)
100
105
100
1:16
Range (%)
93-107
95-110
89-104
Average (%)
99
102
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-97
92
EDTA plasma (n=5)
86-100
93
Cell culture media (n=5)
101-116
108
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
92.65
192.22
797.09
86.02
177.86
741.49
Standard deviation
10.01
19.95
64.09
8.65
18.76
49.16
C V (%)
10.8
10.38
8.04
10.06
10.55
6.63
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat HNF1a concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat HNF1a in samples. No significant cross-reactivity or interference between Rat HNF1a and analogues was observed.
