Description
Rat HNF4a (Hepatocyte Nuclear Factor 4 Alpha) ELISA Kit
The Rat HNF4a (Hepatocyte Nuclear Factor 4 Alpha) ELISA Kit is meticulously crafted for the quantitative assessment of Hepatocyte Nuclear Factor 4 Alpha levels in various biological samples of rat origin. HNF4a is a key transcription factor critical for the regulation of hepatic gene expression, playing a pivotal role in liver development, metabolism, and homeostasis. By accurately measuring HNF4a levels, researchers can gain insights into its function in hepatocytes, impacting processes like glucose and lipid metabolism, cell differentiation, and liver-specific gene expression. Precise detection of Hepatocyte Nuclear Factor 4 Alpha is essential for deciphering its intricate regulatory roles in liver biology and metabolic pathways, furthering our understanding of hepatic function and related disorders.
The Rat HNF4a ELISA Kit offers exceptional sensitivity and specificity, ensuring dependable and reproducible results essential for insightful research and potential therapeutic avenues. Manufactured under stringent quality control measures, this kit ensures robust performance and ease of use, making it a valuable asset for researchers investigating the mechanisms governed by HNF4a in liver-related processes.
| Product Name: | Rat HNF4a (Hepatocyte Nuclear Factor 4 Alpha) ELISA Kit |
| Product Code: | AEES00431 |
| Assay Type: | Sandwich |
| Format: | 96T |
| Assay Time: | 3.5h |
| Reactivity: | Rat |
| Detection Range: | 62.50-4000 pg/mL |
| Sensitivity: | 37.50 pg/mL |
| Sample Type & Sample Volume: | Serum, plasma and other biological fluids, 100?L |
| Specificity: | This kit recognizes Rat HNF4a in samples. No significant cross-reactivity or interference between Rat HNF4a and analogues was observed. |
| Reproducibility: | Both intra-CV and inter-CV are < 10%. |
| Application: | This ELISA kit applies to the in vitro quantitative determination of Rat HNF4a concentrations in serum, plasma and other biological fluids. |
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat HNF4a. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat HNF4a and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat HNF4a, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat HNF4a. You can calculate the concentration of Rat HNF4a in the samples by comparing the OD of the samples to the standard curve.
| Kit Components: | An unopened kit can be stored at 2-8? for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
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| 1. | Add 100?L standard or sample to the wells. Incubate for 90 min at 37°C |
| 2. | Discard the liquid, immediately add 100?L Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C |
| 3. | Aspirate and wash the plate for 3 times |
| 4. | Add 100?L HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times |
| 5. | Add 90?L Substrate Reagent. Incubate for 15 min at 37°C |
| 6. | Add 50?L Stop Solution |
| 7. | Read the plate at 450nm immediately. Calculation of the results |
