The Rat HP (Haptoglobin) CLIA Kit is a reliable tool for measuring haptoglobin levels in rat serum, plasma, and other biological samples. This kit offers exceptional sensitivity and specificity, allowing for accurate and consistent results in a variety of research settings.Haptoglobin is an important protein that binds free hemoglobin, protecting tissues from oxidative damage. Changes in haptoglobin levels have been associated with various diseases, including inflammation, infection, and hemolysis.
This makes the Rat HP CLIA Kit an invaluable resource for researchers studying these conditions and developing potential treatments.Overall, the Rat HP CLIA Kit from AssayGenie is a valuable tool for studying haptoglobin levels in rat samples, providing researchers with the precise data needed to advance their understanding of disease mechanisms and therapeutic interventions.
Product Name:
Rat HP (Haptoglobin) CLIA Kit
SKU:
RTES00273
Target:
Rat HP (Haptoglobin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
1.88 ng/mL
Detection range:
3.13-200 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat HP. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat HP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat HP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat HP. You can calculate the concentration of Rat HP in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
102-118
85-97
95-105
Average (%)
110
92
100
1:4
Range (%)
88-100
101-118
97-111
Average (%)
93
109
105
1:8
Range (%)
86-97
97-114
85-97
Average (%)
92
105
92
1:16
Range (%)
92-104
101-116
98-114
Average (%)
99
109
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-109
99
EDTA plasma (n=5)
89-105
97
Cell culture media (n=5)
100-116
106
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
10.85
19.79
70.89
11.75
19.97
76.67
Standard deviation
1.03
2.33
4.91
1.51
1.59
7.11
C V (%)
9.49
11.77
6.93
12.85
7.96
9.27
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat HP concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat HP in samples. No significant cross-reactivity or interference between Rat HP and analogues was observed.
