The Rat HPA (Heparanase) CLIA Kit is a cutting-edge assay designed for the precise measurement of heparanase levels in rat samples. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results for a variety of research purposes.Heparanase is a key enzyme responsible for breaking down heparan sulfate in the extracellular matrix, playing a critical role in processes such as cell migration, invasion, and angiogenesis.
Dysregulation of heparanase has been implicated in numerous diseases, including cancer, inflammation, and diabetes, making it an important target for studying disease mechanisms and potential therapeutic interventions.With its advanced technology and ease of use, the Rat HPA CLIA Kit is a valuable tool for researchers looking to explore the role of heparanase in rat models and advance our understanding of its implications in various pathologies.
Product Name:
Rat HPA (Heparanase) CLIA Kit
SKU:
RTES00288
Target:
Rat HPA (Heparanase)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat HPA. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat HPA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat HPA, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat HPA. You can calculate the concentration of Rat HPA in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-112
101-112
92-104
Average (%)
103
107
98
1:4
Range (%)
102-118
99-115
95-109
Average (%)
109
107
102
1:8
Range (%)
94-109
97-113
91-104
Average (%)
101
105
97
1:16
Range (%)
87-101
99-110
84-99
Average (%)
92
105
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-102
93
EDTA plasma (n=5)
95-112
103
Cell culture media (n=5)
83-96
90
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
53.26
153.29
411.06
56.15
166.01
407.51
Standard deviation
6.6
18.21
33.05
7.16
16.98
27.1
C V (%)
12.39
11.88
8.04
12.75
10.23
6.65
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat HPA concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat HPA in samples. No significant cross-reactivity or interference between Rat HPA and analogues was observed.
