Rat C-Reactive Protein (CRP) CLIA Kit (High Sensitivity)
The Rat hs-CRP (High Sensitivity C-Reactive Protein) CLIA Kit is a cutting-edge diagnostic tool designed for the precise measurement of high sensitivity C-reactive protein levels in rat serum and plasma samples. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.High sensitivity C-reactive protein is a critical marker of inflammation and is closely associated with various health conditions, including cardiovascular disease, diabetes, and autoimmune disorders.
Monitoring hs-CRP levels can provide valuable insights into the inflammatory response and aid in disease diagnosis and management.Whether investigating the pathogenesis of inflammatory diseases or evaluating therapeutic interventions, the Rat hs-CRP CLIA Kit is an indispensable tool for researchers seeking to deepen their understanding of inflammation and develop novel treatment strategies. Trust in the reliability and performance of this advanced kit to drive your research forward.
Product Name:
Rat C-Reactive Protein (CRP) CLIA Kit (High Sensitivity)
SKU:
RTES00300
Target:
Rat hs-CRP (High Sensitivity C-Reactive Protein)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat hs-CRP. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat hs-CRP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat hs-CRP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat hs-CRP. You can calculate the concentration of Rat hs-CRP in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-112
101-113
95-110
Average (%)
104
107
100
1:4
Range (%)
95-110
96-109
96-112
Average (%)
103
103
103
1:8
Range (%)
85-101
90-104
91-107
Average (%)
92
95
97
1:16
Range (%)
96-114
89-101
98-110
Average (%)
104
95
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-103
95
EDTA plasma (n=5)
99-116
107
Cell culture media (n=5)
95-112
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
108.9
250.07
805.11
119.78
231.93
861.09
Standard deviation
11.95
27.43
58.53
10.05
23.61
59.76
C V (%)
10.97
10.97
7.27
8.39
10.18
6.94
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat hs-CRP concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat hs-CRP in samples. No significant cross-reactivity or interference between Rat hs-CRP and analogues was observed.
