Rat HSF2 (Heat Shock Transcription Factor 2) CLIA Kit
The Rat HSF2 (Heat Shock Transcription Factor 2) CLIA Kit is specially designed for the precise detection of HSF2 levels in rat samples, including serum, plasma, and cell culture supernatants. This kit offers superior sensitivity and specificity, ensuring accurate and consistent results that are ideal for a variety of research purposes.HSF2 is a critical transcription factor involved in the cellular response to heat shock and other stressors. It plays a key role in regulating the expression of heat shock proteins, which are essential for the cell's survival under stressful conditions.
Dysregulation of HSF2 has been implicated in various diseases including cancer, neurodegenerative disorders, and cardiovascular diseases, making it an important biomarker for studying these conditions and developing potential therapeutic interventions.Overall, the Rat HSF2 CLIA Kit provides researchers with a reliable tool for studying the role of HSF2 in disease pathogenesis and for identifying potential targets for therapeutic intervention.
Product Name:
Rat HSF2 (Heat Shock Transcription Factor 2) CLIA Kit
SKU:
RTES00282
Target:
Rat HSF2 (Heat Shock Transcription Factor 2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat HSF2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat HSF2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat HSF2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat HSF2. You can calculate the concentration of Rat HSF2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-109
95-113
94-105
Average (%)
103
103
100
1:4
Range (%)
100-114
94-106
90-102
Average (%)
108
101
97
1:8
Range (%)
90-104
85-101
96-112
Average (%)
97
92
104
1:16
Range (%)
89-102
85-99
94-108
Average (%)
94
92
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-100
94
EDTA plasma (n=5)
96-108
102
Cell culture media (n=5)
91-101
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
101.28
300.75
936.7
106.95
300.05
990.18
Standard deviation
11.2
21.44
104.91
11.87
22.71
105.06
C V (%)
11.06
7.13
11.2
11.1
7.57
10.61
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat HSF2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat HSF2 in samples. No significant cross-reactivity or interference between Rat HSF2 and analogues was observed.
