Description
Rat HSP-60 (Heat Shock Protein 60) ELISA Kit
The Rat HSP-60 (Heat Shock Protein 60) ELISA Kit is a cutting-edge assay designed to quantitatively measure Heat Shock Protein 60 levels in various rat biological samples. Heat shock proteins are crucial molecular chaperones that participate in cellular protection, protein folding, and stress response pathways. HSP-60, specifically, plays a vital role in maintaining cellular homeostasis and promoting cell survival under stressful conditions. This ELISA kit provides researchers with a reliable tool to assess HSP-60 expression levels, offering insights into cellular stress responses, protein quality control mechanisms, and disease pathophysiology.
By accurately quantifying HSP-60, researchers can deepen their understanding of its involvement in cellular processes, stress tolerance, and disease development. Exemplifying exceptional sensitivity and specificity, the Rat HSP-60 ELISA Kit ensures precise and reproducible results, bolstering research outcomes. With robust performance and user-friendly protocols, this kit stands as an excellent choice for scientists investigating the roles of HSP-60 in cellular stress responses, protein homeostasis, and disease conditions.
| Product Name: | Rat HSP-60 (Heat Shock Protein 60) ELISA Kit |
| Product Code: | AEES00424 |
| Assay Type: | Sandwich |
| Format: | 96T |
| Assay Time: | 3.5h |
| Reactivity: | Rat |
| Detection Range: | 0.31-20 ng/mL |
| Sensitivity: | 0.19 ng/mL |
| Sample Type & Sample Volume: | Serum, plasma and other biological fluids, 100?L |
| Specificity: | This kit recognizes Rat HSP-60 in samples. No significant cross-reactivity or interference between Rat HSP-60 and analogues was observed. |
| Reproducibility: | Both intra-CV and inter-CV are < 10%. |
| Application: | This ELISA kit applies to the in vitro quantitative determination of Rat HSP-60 concentrations in Serum, plasma and other biological fluids. |
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat HSP-60. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat HSP-60 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat HSP-60, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat HSP-60. You can calculate the concentration of Rat HSP-60 in the samples by comparing the OD of the samples to the standard curve.
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat HSP-60 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat HSP-60 were tested on 3 different plates, 20 replicates in each plate.
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) | 1.00 | 2.80 | 7.00 | 1.00 | 2.70 | 6.50 |
| Standard deviation | 0.10 | 0.10 | 0.20 | 0.10 | 0.20 | 0.20 |
| CV (%) | 10.00 | 3.57 | 2.86 | 10.00 | 7.41 | 3.08 |
Recovery
The recovery of Rat HSP-60 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 93-104 | 99 |
| EDTA plasma (n=8) | 89-101 | 94 |
| Cell culture media (n=8) | 96-111 | 102 |
| Kit Components: | An unopened kit can be stored at 2-8? for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
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| 1. | Add 100?L standard or sample to the wells. Incubate for 90 min at 37°C |
| 2. | Discard the liquid, immediately add 100?L Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C |
| 3. | Aspirate and wash the plate for 3 times |
| 4. | Add 100?L HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times |
| 5. | Add 90?L Substrate Reagent. Incubate for 15 min at 37°C |
| 6. | Add 50?L Stop Solution |
| 7. | Read the plate at 450nm immediately. Calculation of the results |
