The Rat HSPA4 (Heat Shock 70kDa Protein 4) CLIA Kit is a cutting-edge assay designed for the precise quantification of HSPA4 levels in rat samples. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results for various research purposes.HSPA4, also known as HSP70, is a critical molecular chaperone that plays a key role in protein folding and cellular stress response. Its expression is upregulated in response to environmental stressors, making it a valuable marker for studying cellular stress and protein homeostasis in research areas such as aging, neurodegenerative diseases, and cancer.
With its advanced technology and reliable performance, the Rat HSPA4 CLIA Kit is an essential tool for scientists and researchers looking to explore the functions and mechanisms of HSPA4 in rat models. Unlock new insights into cellular stress and protein quality control with this innovative assay kit.
Product Name:
Rat HSPA4 (Heat Shock 70kDa Protein 4) CLIA Kit
SKU:
RTES00274
Target:
Rat HSPA4 (Heat Shock 70kDa Protein 4)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat HSPA4. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat HSPA4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat HSPA4, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat HSPA4. You can calculate the concentration of Rat HSPA4 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-102
86-100
93-105
Average (%)
97
92
100
1:4
Range (%)
97-109
104-120
100-116
Average (%)
102
110
107
1:8
Range (%)
87-98
96-111
85-99
Average (%)
93
104
92
1:16
Range (%)
99-116
83-97
89-102
Average (%)
107
90
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-100
92
EDTA plasma (n=5)
98-113
106
Cell culture media (n=5)
85-96
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
95.14
292.09
763.89
99.11
314.04
708.54
Standard deviation
11.45
28.74
85.02
9.48
26.29
62.14
C V (%)
12.03
9.84
11.13
9.57
8.37
8.77
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat HSPA4 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat HSPA4 in samples. No significant cross-reactivity or interference between Rat HSPA4 and analogues was observed.
