The Rat HSPG (Heparan Sulphate Proteoglycan) CLIA Kit (RTES00287) is a cutting-edge assay designed for the accurate measurement of HSPG levels in rat samples such as serum, plasma, and tissue lysates. This kit offers superior sensitivity and specificity, allowing researchers to obtain reliable and consistent results for their studies.HSPGs are essential components of the extracellular matrix and play a crucial role in cell adhesion, growth factor signaling, and tissue development. Abnormalities in HSPG expression have been linked to various diseases, including cancer, diabetes, and inflammatory disorders.
Therefore, measuring HSPG levels can provide valuable insights into the pathogenesis of these conditions and help in the development of targeted therapies.With its user-friendly protocol and quick turnaround time, the Rat HSPG CLIA Kit is a valuable tool for researchers investigating the role of HSPGs in health and disease. Trust Assay Genie to deliver high-quality assays for your research needs. Visit the website to learn more about this innovative kit: www.assaygenie.com/rat-hspg-heparan-sulphate-proteoglycan-clia-kit-rtes00287/
Product Name:
Rat HSPG (Heparan Sulphate Proteoglycan) CLIA Kit
SKU:
RTES00287
Target:
Rat HSPG (Heparan Sulphate Proteoglycan)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat HSPG. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat HSPG and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat HSPG, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat HSPG. You can calculate the concentration of Rat HSPG in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-101
99-114
102-117
Average (%)
96
108
108
1:4
Range (%)
85-100
89-100
99-115
Average (%)
92
94
105
1:8
Range (%)
97-110
88-104
100-112
Average (%)
104
95
106
1:16
Range (%)
103-116
91-105
98-113
Average (%)
109
97
106
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
97-112
102
EDTA plasma (n=5)
92-106
98
Cell culture media (n=5)
89-101
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
193.74
506.22
1831.97
176.71
544.75
1979.6
Standard deviation
23.38
57.0
128.42
21.51
52.9
192.02
C V (%)
12.07
11.26
7.01
12.17
9.71
9.7
Sample type &Sample volume:
Tissue homogenates; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat HSPG concentrations in Tissue homogenates.
Specificity:
This kit recognizes Rat HSPG in samples. No significant cross-reactivity or interference between Rat HSPG and analogues was observed.
