The Rat Hyaluronoglucosaminidase-1 (HYAL1) ELISA Kit is a powerful tool for detecting and measuring levels of HYAL1 in rat serum, plasma, and tissue lysates. HYAL1 is an enzyme involved in the breakdown of hyaluronic acid, a key component of the extracellular matrix.Accurate measurement of HYAL1 levels can provide valuable insights into various physiological and pathological processes, including inflammation, tissue remodeling, and cancer progression.
This kit offers high sensitivity and specificity, ensuring precise and reliable results for your research needs.By studying HYAL1 expression and activity, researchers can gain a deeper understanding of its role in disease development and progression, as well as explore its potential as a therapeutic target. Trust the Rat HYAL1 ELISA Kit for robust and reproducible data that can move your research forward.
Product Name:
Rat Hyaluronoglucosaminidase 1 (HYAL1) ELISA Kit
Product Code:
RTDL00517
Size:
96 Assays
Target:
HYAL1
Synonyms:
LUCA1, NAT6, Hyaluronidase-1, Lung carcinoma protein 1
Detection Method:
Sandwich
Range:
1.56-100ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
