SynonymsMTPB,Mitochondrial Trifunctional Protein,Beta Subunit,3-Ketoacyl-Coenzyme A Thiolase/Enoyl-Coenzyme A Hydratase(Trifunctional Protein)Beta Subunit,Beta-ketothiolase
ReactivityRat
Sample TypeTissue Homogenates And Other Biological Fluids
SKU:
RTDL00475
UPC:
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q60587
Sensitivity:
0.135ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
MTPB,Mitochondrial Trifunctional Protein,Beta Subunit,3-Ketoacyl-Coenzyme A Thiolase/Enoyl-Coenzyme A Hydratase(Trifunctional Protein)Beta Subunit,Beta-ketothiolase
Rat Hydroxyacyl Coenzyme A Dehydrogenase Beta (HADHb) ELISA Kit
The Rat Hydroxyacyl-Coenzyme A Dehydrogenase Beta (HADHB) ELISA Kit is specifically designed for the accurate detection of HADHB levels in rat serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.HADHB is an important enzyme involved in fatty acid metabolism, playing a crucial role in energy production and lipid metabolism.
Dysregulation of HADHB has been linked to metabolic disorders such as diabetes, obesity, and fatty liver disease, making it a valuable biomarker for studying these conditions and potential therapeutic targets.Overall, the Rat HADHB ELISA Kit provides researchers with a powerful tool for studying the role of HADHB in metabolic pathways and disease development, offering valuable insights for the development of targeted therapies.
Product Name:
Rat Hydroxyacyl Coenzyme A Dehydrogenase Beta (HADHb) ELISA Kit
Product Code:
RTDL00475
Size:
96 Assays
Target:
HADHb
Synonyms:
MTPB, Mitochondrial Trifunctional Protein,Beta Subunit, 3-Ketoacyl-Coenzyme A Thiolase/Enoyl-Coenzyme A Hydratase(Trifunctional Protein)Beta Subunit, Beta-ketothiolase
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
