Rat IFI16/p16 (Interferon-Inducible Protein 16) CLIA Kit
The Rat IFI16 (P16 Interferon Inducible Protein 16) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of IFI16 levels in rat samples. This kit is reliable and reproducible, providing researchers with valuable insights into the role of IFI16 in immune response and inflammatory processes.IFI16 is a key protein involved in the regulation of immune responses and inflammation, playing a critical role in the body's defense against pathogens and diseases. Dysregulation of IFI16 has been linked to autoimmune disorders, inflammatory diseases, and viral infections, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions.
Overall, the Rat IFI16 CLIA Kit is a powerful tool for researchers looking to investigate the role of IFI16 in various physiological and pathological processes in rat models. Its high sensitivity and specificity ensure accurate and reliable results, making it an excellent choice for a wide range of research applications.
Product Name:
Rat IFI16/p16 (Interferon-Inducible Protein 16) CLIA Kit
SKU:
RTES00330
Target:
Rat IFI16/p16 (Interferon-Inducible Protein 16)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat IFI16/p16. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat IFI16/p16 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IFI16/p16, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat IFI16/p16. You can calculate the concentration of Rat IFI16/p16 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-112
97-110
91-103
Average (%)
104
102
97
1:4
Range (%)
97-114
91-107
89-105
Average (%)
104
98
96
1:8
Range (%)
91-102
93-106
95-108
Average (%)
96
99
101
1:16
Range (%)
100-115
99-115
96-112
Average (%)
105
107
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
101-113
107
EDTA plasma (n=5)
86-96
91
Cell culture media (n=5)
92-103
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
26.86
72.01
200.57
25.79
72.97
201.19
Standard deviation
3.12
8.0
14.92
3.06
5.41
20.58
C V (%)
11.62
11.11
7.44
11.87
7.41
10.23
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat IFI16/p16 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat IFI16/p16 in samples. No significant cross-reactivity or interference between Rat IFI16/p16 and analogues was observed.
