The Rat IFN-β (Interferon Beta) ELISA Kit is a specialized assay crafted for the precise quantitative measurement of Interferon Beta levels in diverse biological samples. Interferon Beta is a key cytokine that plays a pivotal role in the regulation of the immune response, particularly in viral infections and autoimmune diseases. Understanding the levels of Interferon Beta can provide critical insights into the body's immune reaction dynamics and its contribution to various immune-related conditions.
Accurate quantification of IFN-β is essential for unraveling its role in immune function, disease pathogenesis, and the development of targeted therapeutic interventions. With exceptional sensitivity and specificity, Assay Genie's IFN-β ELISA Kit offers reliable and reproducible results. Manufactured under stringent quality control standards, this kit ensures robust performance, making it a valuable tool for cutting-edge research in immunology and infectious diseases.
Product Name:
Rat IFN-beta (Interferon Beta) ELISA Kit
SKU:
AEES00448
Target:
Rat IFN-β (Interferon Beta)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat IFN-β. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat IFN-β and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IFN-β, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat IFN-β. You can calculate the concentration of Rat IFN-β in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-100
91-106
97-112
Average (%)
92
98
105
1:4
Range (%)
98-112
79-94
94-109
Average (%)
106
86
101
1:8
Range (%)
102-118
81-94
96-112
Average (%)
108
86
104
1:16
Range (%)
95-111
82-97
95-111
Average (%)
103
89
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-99
94
EDTA plasma (n=5)
92-108
99
Cell culture media (n=5)
87-101
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
49.2
157.9
381.5
47.6
170.3
360.4
Standard deviation
2.7
8.1
18.7
3.1
7.3
10.8
C V (%)
5.49
5.13
4.9
6.51
4.29
3.0
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat IFN-β concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat IFN-β in samples. No significant cross-reactivity or interference between Rat IFN-β and analogues was observed.
