Rat IGFBP-4 (Insulin-Like Growth Factor Binding Protein 4) CLIA Kit
The Rat IGFBP-4 (Insulin-like Growth Factor Binding Protein 4) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of IGFBP-4 levels in rat serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it an invaluable tool for a variety of research applications.IGFBP-4 is a key protein involved in regulating the bioavailability and activity of insulin-like growth factors. It plays a crucial role in modulating cell growth, differentiation, and apoptosis, making it a critical player in various physiological and pathological processes.
By accurately measuring IGFBP-4 levels, researchers can gain valuable insights into the mechanisms underlying growth regulation and potential therapeutic targets for conditions such as cancer, diabetes, and metabolic disorders.Overall, the Rat IGFBP-4 CLIA Kit offers researchers a powerful tool for studying the role of IGFBP-4 in health and disease, providing accurate and precise measurements to support their scientific investigations.
Product Name:
Rat IGFBP-4 (Insulin-Like Growth Factor Binding Protein 4) CLIA Kit
SKU:
RTES00319
Target:
Rat IGFBP-4 (Insulin-Like Growth Factor Binding Protein 4)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
0.09 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat IGFBP-4. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat IGFBP-4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IGFBP-4, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat IGFBP-4. You can calculate the concentration of Rat IGFBP-4 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-118
90-104
84-96
Average (%)
108
96
90
1:4
Range (%)
91-108
83-95
86-99
Average (%)
99
90
91
1:8
Range (%)
85-96
85-99
89-103
Average (%)
91
92
95
1:16
Range (%)
90-104
89-103
95-112
Average (%)
96
95
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-111
102
EDTA plasma (n=5)
95-108
101
Cell culture media (n=5)
93-109
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.5
1.52
4.94
0.54
1.45
4.93
Standard deviation
0.05
0.16
0.36
0.05
0.1
0.36
C V (%)
10.0
10.53
7.29
9.26
6.9
7.3
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat IGFBP-4 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat IGFBP-4 in samples. No significant cross-reactivity or interference between Rat IGFBP-4 and analogues was observed.
