Rat IKB alpha (Inhibitory Subunit of NF-kB alpha) CLIA Kit
The Rat IKB Alpha (Inhibitory Subunit of NF-KB Alpha) CLIA Kit is a highly reliable assay designed for the sensitive and accurate quantification of IKB Alpha levels in rat samples. This advanced kit offers excellent specificity and precision, ensuring consistent and trustworthy results for a variety of research applications.IKB Alpha is a key regulator of the NF-KB signaling pathway, playing a critical role in inflammation, immune response, and cell survival. Dysregulation of IKB Alpha has been linked to various diseases, including cancer, autoimmune disorders, and inflammatory conditions.
Therefore, measuring IKB Alpha levels can provide valuable insights into disease mechanisms and potential therapeutic targets.With its robust performance and user-friendly protocol, the Rat IKB Alpha CLIA Kit is an indispensable tool for researchers studying NF-KB signaling, inflammation, and related pathways in rat models. Trust in this kit to deliver precise and reproducible data for your research needs.
Product Name:
Rat IKB alpha (Inhibitory Subunit of NF-kB alpha) CLIA Kit
SKU:
RTES00311
Target:
Rat IKB alpha (Inhibitory Subunit of NF-kB alpha)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat IKBα. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat IKBα and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IKBα, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat IKBα. You can calculate the concentration of Rat IKBα in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-106
94-109
100-115
Average (%)
100
100
108
1:4
Range (%)
90-104
99-112
85-98
Average (%)
98
104
92
1:8
Range (%)
102-118
98-113
100-117
Average (%)
108
104
109
1:16
Range (%)
98-112
87-102
101-114
Average (%)
104
93
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
98-111
104
EDTA plasma (n=5)
95-109
103
Cell culture media (n=5)
97-112
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
182.02
410.64
1626.83
168.81
374.94
1560.72
Standard deviation
18.89
37.9
162.19
15.29
35.51
112.53
C V (%)
10.38
9.23
9.97
9.06
9.47
7.21
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat IKBα concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat IKBα in samples. No significant cross-reactivity or interference between Rat IKBα and analogues was observed.
