The Rat IL-18 ELISA Kit is engineered to facilitate the precise and quantitative measurement of Interleukin-18 (IL-18) levels in a variety of rat biological specimens. IL-18, a crucial pro-inflammatory cytokine, plays a pivotal role in a broad spectrum of cellular functions, including immune response modulation, cell proliferation, and inflammatory signaling pathways.
Its involvement extends to regulating inflammatory cytokine cascades and immune cell activation processes. Crafted by Assay Genie, this IL-18 ELISA Kit ensures remarkable sensitivity and specificity, enabling the generation of accurate and reproducible data. With a focus on stringent quality control benchmarks, this kit assures robust performance and a user-friendly experience, making it an indispensable asset for researchers delving into the intricate roles of IL-18 in diverse physiological contexts.
Product Name:
Rat IL-18 ELISA Kit
SKU:
AEES00458
Target:
Rat IL-18
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat IL-18. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat IL-18 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IL-18, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat IL-18. You can calculate the concentration of Rat IL-18 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-106
90-106
86-98
Average (%)
99
97
91
1:4
Range (%)
99-111
85-101
97-110
Average (%)
105
92
104
1:8
Range (%)
97-111
87-98
96-112
Average (%)
103
93
103
1:16
Range (%)
102-119
83-97
96-112
Average (%)
108
90
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-100
91
EDTA plasma (n=5)
95-108
101
Cell culture media (n=5)
88-102
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
52
118.3
412.0
56.7
128.4
387.3
Standard deviation
3.6
4.9
21.0
3.1
6.5
12.8
C V (%)
6.92
4.14
5.1
5.47
5.06
3.3
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat IL-18 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat IL-18 in samples. No significant cross-reactivity or interference between Rat IL-18 and analogues was observed.
