The Rat IL-2 ELISA Kit is a specialized assay developed for the quantitative measurement of Interleukin-2 (IL-2) levels in diverse rat biological samples.
IL-2 is a pivotal cytokine that plays a crucial role in immune regulation, particularly in the activation and proliferation of T cells. Its involvement in modulating immune responses and coordinating cellular interactions underscores its significance in various disease conditions, autoimmune disorders, and infections. Accurate detection of IL-2 levels is essential for unraveling the intricate mechanisms of immune function and for designing precise therapeutic interventions. Our IL-1 ELISA Kit is meticulously crafted to deliver superior sensitivity and specificity, ensuring precise and reproducible results. Manufactured under stringent quality control measures, this kit offers robust performance and user-friendly protocols, making it an indispensable tool for researchers aiming to investigate the functions and impact of IL-2 in immunological processes.
Product Name:
Rat IL-2 ELISA Kit
SKU:
AEES00295
Target:
Rat IL-2
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat IL-2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat IL-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IL-2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat IL-2. You can calculate the concentration of Rat IL-2 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-108
97-113
92-106
Average (%)
100
104
98
1:4
Range (%)
98-112
85-96
94-110
Average (%)
106
90
101
1:8
Range (%)
101-114
80-91
94-107
Average (%)
108
87
99
1:16
Range (%)
99-112
82-95
94-104
Average (%)
106
88
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-105
99
EDTA plasma (n=5)
95-109
102
Cell culture media (n=5)
93-110
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
109.4
281.1
992.7
106.2
286.2
1010.3
Standard deviation
5.8
14.1
40.7
6.8
17.2
53.5
C V (%)
5.3
5.02
4.1
6.4
6.01
5.3
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat IL-2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat IL-2 in samples. No significant cross-reactivity or interference between Rat IL-2 and analogues was observed.
