The Rat IL-23 ELISA Kit is precisely engineered to quantify Interleukin-23 (IL-23) in diverse rat biological samples, offering a comprehensive and accurate analytical solution.
IL-23, a vital proinflammatory cytokine, plays a crucial role in various cellular processes, including immune response modulation, cell differentiation, and regulation of inflammatory cascades. Its functions are integral to orchestrating immune system responses and have implications in numerous inflammatory disorders and autoimmunity. Developed by Assay Genie, this IL-23 ELISA Kit ensures unparalleled sensitivity and specificity, delivering consistent and reliable results. Crafted under stringent quality controls, this kit provides robust performance and user-friendly operation, making it an indispensable tool for researchers investigating the intricate roles of IL-23 in diverse biological contexts.
Product Name:
Rat IL-23 ELISA Kit
SKU:
AEES00459
Target:
Rat IL-23
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat IL-23. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat IL-23 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IL-23, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat IL-23. You can calculate the concentration of Rat IL-23 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-111
94-106
98-112
Average (%)
105
100
105
1:4
Range (%)
91-105
83-95
85-95
Average (%)
97
88
90
1:8
Range (%)
87-98
85-99
86-100
Average (%)
92
92
93
1:16
Range (%)
88-100
83-95
83-98
Average (%)
93
88
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-101
95
EDTA plasma (n=5)
87-100
93
Cell culture media (n=5)
94-108
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
47.13
117.24
381.16
46.32
108.46
389.05
Standard deviation
2.66
6.39
20.62
2.85
4.78
12.57
C V (%)
5.64
5.45
5.41
6.15
4.41
3.23
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat IL-23 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat IL-23 in samples. No significant cross-reactivity or interference between Rat IL-23 and analogues was observed.
