The Rat IL1RA (Interleukin 1 Receptor Antagonist) CLIA Kit is a powerful tool designed for the precise measurement of IL1RA levels in rat samples, including serum, plasma, and cell culture supernatants. This kit offers superior sensitivity and specificity, ensuring accurate and reliable results for a variety of research applications.IL1RA is a key regulator of inflammation and immune responses, playing a crucial role in various disease states, including autoimmune disorders, inflammatory conditions, and infectious diseases.
By accurately measuring IL1RA levels, researchers can gain valuable insights into the mechanisms underlying these diseases and potentially develop targeted therapies for treatment.Overall, the Rat IL1RA CLIA Kit is an essential tool for researchers studying inflammation, immunity, and disease pathology in rats, providing valuable data for advancing our understanding of these complex biological processes.
Product Name:
Rat IL-1ra CLIA Kit
SKU:
RTES00332
Target:
Rat IL-1ra
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat IL-1ra. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat IL-1ra and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IL-1ra, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat IL-1ra. You can calculate the concentration of Rat IL-1ra in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
101-115
103-120
90-105
Average (%)
107
109
96
1:4
Range (%)
84-96
89-102
90-106
Average (%)
91
95
98
1:8
Range (%)
103-116
94-107
90-102
Average (%)
110
100
96
1:16
Range (%)
102-119
96-109
90-106
Average (%)
110
101
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-110
101
EDTA plasma (n=5)
86-98
92
Cell culture media (n=5)
85-96
90
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
50.89
88.67
477.77
54.03
84.16
523.3
Standard deviation
4.46
6.56
32.82
4.42
9.7
33.54
C V (%)
8.76
7.4
6.87
8.18
11.53
6.41
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat IL-1ra concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat IL-1ra in samples. No significant cross-reactivity or interference between Rat IL-1ra and analogues was observed.
