The Rat IL-2SR alpha (CD25) ELISA Kit is specifically designed for the precise measurement of interleukin-2 receptor alpha levels in rat samples including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for various research applications.Interleukin-2 receptor alpha is a key protein involved in immune responses, regulating T-cell activation and proliferation. Dysregulation of IL-2SR alpha has been linked to various immune-related diseases, making it a valuable biomarker for studying autoimmune disorders, inflammation, and infectious diseases.
With its user-friendly protocol and reliable performance, the Rat IL-2SR alpha ELISA Kit is an essential tool for research studies focusing on immune responses and related pathologies in rat models. Get reliable and reproducible results with this high-quality ELISA kit from Assay Genie.
Product Name:
Rat IL-2SR alpha ELISA Kit
SKU:
RTES00749
Target:
Rat IL-2SR alpha
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat IL-2sRα/CD25. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat IL-2sRα/CD25 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IL-2sRα/CD25, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat IL-2sRα/CD25. You can calculate the concentration of Rat IL-2sRα/CD25 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-100
88-100
90-103
Average (%)
92
95
96
1:4
Range (%)
86-101
84-96
83-96
Average (%)
93
90
90
1:8
Range (%)
88-99
85-98
86-99
Average (%)
93
92
93
1:16
Range (%)
90-106
81-93
82-96
Average (%)
97
86
89
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-103
98
EDTA plasma (n=5)
91-106
98
Cell culture media (n=5)
88-99
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
273.39
756.15
2135.17
266.5
778.27
2081.5
Standard deviation
16.92
36.67
89.04
14.92
32.14
82.64
C V (%)
6.19
4.85
4.17
5.6
4.13
3.97
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat IL-2sRα/CD25 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat IL-2sRα/CD25 in samples. No significant cross-reactivity or interference between Rat IL-2sRα/CD25 and analogues was observed.
