The Rat INHA (Inhibin A) CLIA Kit is a highly sensitive and specific assay designed for the accurate measurement of inhibin A levels in rat samples. This kit is ideal for researchers studying reproductive health, as inhibin A is a key hormone involved in the regulation of follicle-stimulating hormone (FSH) secretion and plays a critical role in the ovarian cycle.Inhibin A is produced by the ovaries and plays a crucial role in the feedback mechanism that regulates FSH secretion from the pituitary gland.
Dysregulation of inhibin A levels has been linked to conditions such as polycystic ovary syndrome (PCOS), infertility, and ovarian cancer.With its high sensitivity and reliability, the Rat INHA CLIA Kit is a valuable tool for studying reproductive health and developing potential therapies for related conditions. Get accurate and reproducible results with this trusted assay kit from Assay Genie.
Product Name:
Rat INHA (Inhibin A) CLIA Kit
SKU:
RTES00308
Target:
Rat INHA (Inhibin A)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat INHA. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat INHA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat INHA, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat INHA. You can calculate the concentration of Rat INHA in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-105
94-106
88-100
Average (%)
99
101
95
1:4
Range (%)
89-102
89-102
99-113
Average (%)
96
96
106
1:8
Range (%)
102-118
85-96
102-117
Average (%)
109
91
108
1:16
Range (%)
93-108
95-110
97-111
Average (%)
99
102
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-109
101
EDTA plasma (n=5)
97-113
103
Cell culture media (n=5)
90-104
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
25.42
60.4
232.84
27.48
55.2
216.14
Standard deviation
3.04
5.36
24.33
2.34
5.0
23.75
C V (%)
11.96
8.87
10.45
8.52
9.06
10.99
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat INHA concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat INHA in samples. No significant cross-reactivity or interference between Rat INHA and analogues was observed.
