The Rat INHBC (Inhibin Beta-C) CLIA Kit is specifically designed for the accurate measurement of inhibin beta-C levels in rat serum, plasma, and tissue culture samples. This kit offers high sensitivity and specificity, ensuring precise and reliable results for a variety of research applications.Inhibin beta-C is a key regulator of the reproductive system, playing a vital role in follicle development and hormone secretion. Dysregulation of inhibin beta-C has been implicated in conditions such as infertility, polycystic ovary syndrome, and certain types of cancers, highlighting its importance as a biomarker for studying these diseases and potential therapeutic interventions.
Overall, the Rat INHBC CLIA Kit provides researchers with a valuable tool for studying the role of inhibin beta-C in various physiological and pathological processes, ultimately advancing our understanding of reproductive health and disease.
Product Name:
Rat INHbC (Inhibin Beta C) CLIA Kit
SKU:
RTES00309
Target:
Rat INHbC (Inhibin Beta C)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
1.88 pg/mL
Detection range:
3.13-200 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat INHbC. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat INHbC and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat INHbC, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat INHbC. You can calculate the concentration of Rat INHbC in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-98
97-111
95-110
Average (%)
91
105
102
1:4
Range (%)
87-97
93-107
98-110
Average (%)
92
99
104
1:8
Range (%)
87-102
94-107
89-103
Average (%)
95
101
96
1:16
Range (%)
90-104
93-106
102-117
Average (%)
97
99
109
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
100-114
108
EDTA plasma (n=5)
97-114
104
Cell culture media (n=5)
94-107
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
10.67
21.53
90.2
10.99
23.65
94.54
Standard deviation
1.03
2.32
8.17
1.22
2.75
7.81
C V (%)
9.65
10.78
9.06
11.1
11.63
8.26
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat INHbC concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat INHbC in samples. No significant cross-reactivity or interference between Rat INHbC and analogues was observed.
