The Rat INHBP (Inhibin Binding Protein) CLIA Kit is specifically designed for the accurate measurement of inhibin binding protein levels in rat samples. This kit offers high sensitivity and specificity, ensuring precise and reliable results for various research applications.INHBP is a key protein involved in inhibin signaling and regulation, playing a crucial role in reproductive health and hormone balance. Dysregulation of this protein has been linked to various reproductive disorders and conditions, making it a valuable biomarker for studying and understanding these diseases.
With this CLIA kit, researchers can effectively measure and analyze INHBP levels in rat samples, providing valuable insights into reproductive health and potential therapeutic targets. Ideal for studies in reproductive biology, endocrinology, and hormone regulation, the Rat INHBP CLIA Kit is a valuable tool for advancing research in these fields.
Product Name:
Rat INHBP (Inhibin Binding Protein) CLIA Kit
SKU:
RTES00310
Target:
Rat INHBP (Inhibin Binding Protein)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.09 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat INHBP. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat INHBP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat INHBP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat INHBP. You can calculate the concentration of Rat INHBP in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-96
89-101
88-103
Average (%)
90
94
94
1:4
Range (%)
99-113
96-109
102-114
Average (%)
105
102
108
1:8
Range (%)
94-108
84-98
90-103
Average (%)
102
90
97
1:16
Range (%)
103-119
95-107
84-97
Average (%)
109
101
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-101
93
EDTA plasma (n=5)
86-100
92
Cell culture media (n=5)
94-106
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.51
0.96
3.57
0.49
0.88
3.62
Standard deviation
0.06
0.11
0.34
0.06
0.07
0.23
C V (%)
11.76
11.46
9.52
12.24
7.95
6.35
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat INHBP concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat INHBP in samples. No significant cross-reactivity or interference between Rat INHBP and analogues was observed.
