The Rat Inhibin A (INHA) ELISA Kit is a highly reliable and sensitive assay designed for the accurate detection of Inhibin A levels in rat serum, plasma, and cell culture supernatants. This kit is specifically optimized for research involving inhibin A, a key protein that plays a critical role in regulating reproductive function and hormone production in rats.Inhibin A is known for its inhibitory effects on follicle-stimulating hormone (FSH) secretion and is essential for proper ovarian function and hormone balance in rats. Dysregulation of inhibin A levels has been associated with various reproductive disorders and diseases, making it a valuable biomarker for studying reproductive health and potential therapeutic interventions.
With its high sensitivity and specificity, the Rat Inhibin A (INHA) ELISA Kit offers accurate and reproducible results, making it an indispensable tool for researchers and scientists working in the fields of reproductive biology, endocrinology, and hormone regulation. Order your kit today and unlock valuable insights into inhibin A biology in rat models.
Product Name:
Rat Inhibin A (INHA) ELISA Kit
Product Code:
RTDL00575
Size:
96 Assays
Target:
INHA
Synonyms:
INH-A
Detection Method:
Sandwich
Range:
15.625-1,000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
