The Rat Interleukin-34 (IL-34) ELISA Kit is a highly sensitive and specific assay designed to accurately detect interleukin-34 levels in rat serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it an essential tool for researchers studying the role of IL-34 in various biological processes.Interleukin-34 is a cytokine that plays a crucial role in regulating immune responses, cell differentiation, and bone metabolism. Aberrant levels of IL-34 have been implicated in various diseases, including inflammatory disorders, autoimmune conditions, and cancer.
By measuring IL-34 levels, researchers can gain valuable insights into the pathogenesis of these diseases and identify potential therapeutic targets.The Rat Interleukin-34 (IL-34) ELISA Kit is easy to use and requires minimal sample volume, making it suitable for high-throughput screening and large-scale studies. With its high sensitivity and specificity, this kit is an indispensable tool for investigating the biological functions of IL-34 and its potential as a therapeutic target in disease treatment.
Product Name:
Rat Interleukin 34 (IL-34) ELISA Kit
Product Code:
RTDL00565
Size:
96 Assays
Target:
IL34
Synonyms:
C16orf77
Detection Method:
Sandwich
Range:
15.625-1000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
91-98
94
Heparin plasma (n=5)
90-99
94
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
