The Rat ITLN1 (Intelectin-1/Omentin) CLIA Kit is specifically designed for the precise measurement of ITLN1 levels in rat serum, plasma, and cell culture supernatants. This kit boasts a high level of sensitivity and specificity, ensuring accurate and reproducible results for various research applications.Intelectin-1, also known as Omentin, is a key protein involved in various physiological processes such as glucose metabolism, inflammation, and adipogenesis. It plays a critical role in conditions like obesity, insulin resistance, and inflammatory diseases, making it a valuable biomarker for studying these conditions and potential therapeutic interventions.
With the Rat ITLN1 CLIA Kit, researchers can obtain reliable data on ITLN1 levels in rat samples, providing valuable insights into the mechanisms underlying these physiological processes and diseases. Enhance your research with this advanced kit designed to streamline your analysis and yield meaningful results.
Product Name:
Rat ITLN1 (Intelectin 1/Omentin) CLIA Kit
SKU:
RTES00415
Target:
Rat ITLN1 (Intelectin 1/Omentin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat ITLN1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat ITLN1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat ITLN1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat ITLN1. You can calculate the concentration of Rat ITLN1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
102-117
101-113
93-106
Average (%)
108
107
101
1:4
Range (%)
93-108
100-114
90-105
Average (%)
100
106
97
1:8
Range (%)
96-110
85-99
87-101
Average (%)
104
92
92
1:16
Range (%)
101-114
101-116
96-107
Average (%)
108
108
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-99
94
EDTA plasma (n=5)
97-114
105
Cell culture media (n=5)
91-105
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
210.33
502.62
1711.71
228.63
543.26
1834.49
Standard deviation
21.29
48.96
116.4
28.97
42.16
129.7
C V (%)
10.12
9.74
6.8
12.67
7.76
7.07
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat ITLN1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat ITLN1 in samples. No significant cross-reactivity or interference between Rat ITLN1 and analogues was observed.
