Description
Rat ITLN1 (Intelectin 1/Omentin) ELISA Kit
The Rat ITLN1 (Intelectin 1/Omentin) ELISA Kit is meticulously developed to quantitatively measure the levels of ITLN1 in various rat biological samples. Intelectin 1, also known as Omentin, is a distinct adipokine with emerging significance in metabolic regulation, insulin sensitivity, and inflammation.
Its role in modulating metabolic processes, immune responses, and the pathophysiology of metabolic disorders makes ITLN1 a valuable target for investigations into metabolic health, obesity-related complications, and beyond. Accurate quantification of ITLN1 levels through this ELISA kit provides researchers with a deeper insight into the regulatory functions of Intelectin 1 in metabolic homeostasis, adipose tissue biology, and inflammatory signaling pathways. By elucidating the molecular interplay involving ITLN1, researchers can glean insights into its potential as a biomarker for metabolic health assessment and a therapeutic target in metabolic disorders. The Rat ITLN1 ELISA Kit by Assay Genie delivers exceptional sensitivity and specificity, ensuring precise and reproducible outcomes. Manufactured under rigorous quality standards, this kit epitomizes robust performance and user-friendly operation, making it a premier choice for research endeavors aiming to unravel the intricate roles of ITLN1 in metabolic physiology and its implications for health and disease.
| Product Name: | Rat ITLN1 (Intelectin 1/Omentin) ELISA Kit |
| Product Code: | AEES00499 |
| Assay Type: | Sandwich |
| Format: | 96T |
| Assay Time: | 3.5h |
| Reactivity: | Rat |
| Detection Range: | 0.31-20 ng/mL |
| Sensitivity: | 0.19 ng/mL |
| Sample Type & Sample Volume: | Serum, plasma and other biological fluids, 100?L |
| Specificity: | This kit recognizes Rat ITLN1 in samples. No significant cross-reactivity or interference between Rat ITLN1 and analogues was observed. |
| Reproducibility: | Both intra-CV and inter-CV are < 10%. |
| Application: | This ELISA kit applies to the in vitro quantitative determination of Rat ITLN1 concentrations in Serum, plasma and other biological fluids. |
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat ITLN1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat ITLN1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat ITLN1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat ITLN1. You can calculate the concentration of Rat ITLN1 in the samples by comparing the OD of the samples to the standard curve.
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat ITLN1 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat ITLN1 were tested on 3 different plates, 20 replicates in each plate.
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) | 1.03 | 2.70 | 9.02 | 1.13 | 2.50 | 8.61 |
| Standard deviation | 0.06 | 0.14 | 0.29 | 0.08 | 0.13 | 0.35 |
| CV (%) | 5.83 | 5.19 | 3.22 | 7.08 | 5.20 | 4.07 |
Recovery
The recovery of Rat ITLN1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 88-102 | 95 |
| EDTA plasma (n=8) | 91-102 | 97 |
| Cell culture media (n=8) | 88-99 | 93 |
| Kit Components: | An unopened kit can be stored at 2-8? for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
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| 1. | Add 100?L standard or sample to the wells. Incubate for 90 min at 37°C |
| 2. | Discard the liquid, immediately add 100?L Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C |
| 3. | Aspirate and wash the plate for 3 times |
| 4. | Add 100?L HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times |
| 5. | Add 90?L Substrate Reagent. Incubate for 15 min at 37°C |
| 6. | Add 50?L Stop Solution |
| 7. | Read the plate at 450nm immediately. Calculation of the results |
