Rat Kelch Like ECH Associated Protein 1 (KEAP1) ELISA Kit
The Rat KEAP1 (Kelch-like ECH-associated protein 1) ELISA Kit is specially designed for the precise measurement of KEAP1 levels in rat serum, plasma, and cell culture supernatants. This kit ensures accurate and consistent results, with high sensitivity and specificity, making it ideal for a variety of research applications.KEAP1 is a key protein involved in cellular defense against oxidative stress and plays a crucial role in regulating the antioxidant response by controlling the activity of transcription factor NRF2.
Dysregulation of KEAP1-NRF2 signaling has been linked to various diseases, including cancer, neurodegenerative disorders, and inflammatory conditions, making KEAP1 an important biomarker for studying these diseases and potential therapeutic interventions.This ELISA kit provides researchers with a reliable tool to quantitatively measure KEAP1 levels in rat samples, facilitating research in the field of oxidative stress, antioxidant defense mechanisms, and related diseases.
Product Name:
Rat Kelch Like ECH Associated Protein 1 (KEAP1) ELISA Kit
Product Code:
RTDL00601
Size:
96 Assays
Target:
KEAP1
Synonyms:
INrf2, KLHL19, Cytosolic inhibitor of Nrf2, Kelch-like protein 19
Detection Method:
Sandwich
Range:
31.2-2000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
