The Rat LIF (Leukemia Inhibitory Factor) CLIA Kit is a highly reliable and sensitive assay designed for the quantification of LIF levels in rat serum, plasma, and cell culture supernatants. This kit provides precise and accurate results, making it an ideal tool for researchers studying the role of LIF in various biological processes.Leukemia Inhibitory Factor is a cytokine that plays a key role in regulating cell growth and differentiation. It is involved in diverse physiological and pathological processes, including embryonic development, immune response, and cancer progression.
The Rat LIF CLIA Kit allows for the reliable measurement of LIF levels, thereby facilitating the study of its impact on different disease states and potential therapeutic interventions.Overall, the Rat LIF CLIA Kit offers a valuable tool for researchers interested in investigating the biological functions of LIF and its implications in various health conditions. Its high sensitivity and specificity ensure accurate and reproducible results, making it a trusted choice for biomarker analysis and research applications.
Product Name:
Rat LIF (Leukemia Inhibitory Factor) CLIA Kit
SKU:
RTES00361
Target:
Rat LIF (Leukemia Inhibitory Factor)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat LIF. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat LIF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat LIF, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat LIF. You can calculate the concentration of Rat LIF in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-112
87-102
97-113
Average (%)
105
93
103
1:4
Range (%)
93-105
88-102
90-103
Average (%)
98
94
97
1:8
Range (%)
101-118
102-119
86-101
Average (%)
108
110
93
1:16
Range (%)
86-98
93-105
90-105
Average (%)
92
99
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
100-113
106
EDTA plasma (n=5)
100-113
105
Cell culture media (n=5)
85-98
90
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
22.94
45.59
238.15
21.57
42.85
261.24
Standard deviation
2.27
4.37
16.74
1.73
4.37
28.19
C V (%)
9.9
9.59
7.03
8.02
10.2
10.79
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat LIF concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat LIF in samples. No significant cross-reactivity or interference between Rat LIF and analogues was observed.
