Description
Rat LIF (Leukemia Inhibitory Factor) ELISA Kit
The Rat LIF (Leukemia Inhibitory Factor) ELISA Kit is a specialized assay developed to accurately quantify the levels of Leukemia Inhibitory Factor in rat samples. LIF is a multifunctional cytokine that plays a crucial role in cellular proliferation, differentiation, and apoptosis. It is involved in various biological processes, including embryonic development, neurogenesis, and immune response modulation. As a key regulator of cell growth and differentiation, precise measurement of LIF levels is vital for understanding its diverse functions in normal physiology and disease conditions.
Our ELISA kit offers exceptional sensitivity and specificity, ensuring dependable and reproducible results for your research needs. Manufactured under rigorous quality control measures, this kit guarantees robust performance and ease of use, making it a valuable tool for investigations into the complex roles of LIF in cellular activities and molecular pathways. Partner with Assay Genie for high-quality ELISA kits that deliver accurate and insightful data for your scientific inquiries.
| Product Name: | Rat LIF (Leukemia Inhibitory Factor) ELISA Kit |
| Product Code: | AEES00467 |
| Assay Type: | Sandwich |
| Format: | 96T |
| Assay Time: | 3.5h |
| Reactivity: | Rat |
| Detection Range: | 15.63-1000 pg/mL |
| Sensitivity: | 9.38 pg/mL |
| Sample Type & Sample Volume: | Serum, plasma and other biological fluids, 100?L |
| Specificity: | This kit recognizes Rat LIF in samples. No significant cross-reactivity or interference between Rat LIF and analogues was observed. |
| Reproducibility: | Both intra-CV and inter-CV are < 10%. |
| Application: | This ELISA kit applies to the in vitro quantitative determination of Rat LIF concentrations in Serum, plasma and other biological fluids. |
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat LIF. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat LIF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat LIF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat LIF. You can calculate the concentration of Rat LIF in the samples by comparing the OD of the samples to the standard curve.
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat LIF were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat LIF were tested on 3 different plates, 20 replicates in each plate.
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 53.75 | 100.83 | 406.32 | 49.50 | 107.19 | 383.72 |
| Standard deviation | 3.35 | 5.76 | 13.81 | 2.97 | 6.03 | 14.81 |
| CV (%) | 6.23 | 5.71 | 3.40 | 6.00 | 5.63 | 3.86 |
Recovery
The recovery of Rat LIF spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 89-106 | 96 |
| EDTA plasma (n=8) | 90-105 | 97 |
| Cell culture media (n=8) | 87-99 | 94 |
| Kit Components: | An unopened kit can be stored at 2-8? for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
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| 1. | Add 100?L standard or sample to the wells. Incubate for 90 min at 37°C |
| 2. | Discard the liquid, immediately add 100?L Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C |
| 3. | Aspirate and wash the plate for 3 times |
| 4. | Add 100?L HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times |
| 5. | Add 90?L Substrate Reagent. Incubate for 15 min at 37°C |
| 6. | Add 50?L Stop Solution |
| 7. | Read the plate at 450nm immediately. Calculation of the results |
