Rat LIFR (Leukemia Inhibitory Factor Receptor) CLIA Kit
The Rat LIFR (Leukemia Inhibitory Factor Receptor) CLIA Kit is a highly reliable and precise tool designed for the detection of LIFR levels in rat samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results that are essential for a wide range of research applications.Leukemia Inhibitory Factor Receptor (LIFR) is a critical protein involved in various cellular processes, including cell differentiation, proliferation, and survival.
Dysregulation of LIFR has been implicated in numerous diseases, such as leukemia, inflammatory disorders, and neurodegenerative diseases, making it a valuable biomarker for studying these conditions and potentially developing novel therapeutic interventions.Overall, the Rat LIFR CLIA Kit provides researchers with a sophisticated tool to investigate the role of LIFR in different biological pathways and diseases, ultimately leading to a deeper understanding of its function and potential clinical significance.
Product Name:
Rat LIFR (Leukemia Inhibitory Factor Receptor) CLIA Kit
SKU:
RTES00360
Target:
Rat LIFR (Leukemia Inhibitory Factor Receptor)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat LIFR. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat LIFR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat LIFR, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat LIFR. You can calculate the concentration of Rat LIFR in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-114
95-110
92-107
Average (%)
106
101
98
1:4
Range (%)
103-119
96-111
99-113
Average (%)
110
102
104
1:8
Range (%)
86-98
99-111
90-104
Average (%)
92
105
96
1:16
Range (%)
83-98
90-101
86-97
Average (%)
90
95
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
97-112
102
EDTA plasma (n=5)
97-109
104
Cell culture media (n=5)
90-102
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
202.39
415.89
1861.79
184.3
430.63
1799.94
Standard deviation
26.01
43.29
163.09
22.06
43.28
119.16
C V (%)
12.85
10.41
8.76
11.97
10.05
6.62
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat LIFR concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat LIFR in samples. No significant cross-reactivity or interference between Rat LIFR and analogues was observed.
