Rat LOX-1 (Lectin Like Oxidized Low Density Lipoprotein Receptor 1) CLIA Kit
The Rat LOX-1 (Lectin-Like Oxidized Low-Density Lipoprotein Receptor 1) CLIA Kit is a powerful tool for measuring LOX-1 levels in rat samples, including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and reliable results for a variety of research applications.LOX-1 is a key receptor involved in the uptake of oxidized low-density lipoprotein (LDL) in cells, playing a critical role in the development of atherosclerosis and cardiovascular disease.
Elevated levels of LOX-1 have been associated with these conditions, making it a valuable biomarker for understanding disease progression and evaluating potential therapeutic interventions.By using the Rat LOX-1 CLIA Kit, researchers can gain valuable insights into the role of LOX-1 in cardiovascular health and disease, paving the way for new discoveries and advancements in the field of lipid metabolism and cardiovascular research.
Product Name:
Rat LOX-1 (Lectin Like Oxidized Low Density Lipoprotein Receptor 1) CLIA Kit
SKU:
RTES00365
Target:
Rat LOX-1 (Lectin Like Oxidized Low Density Lipoprotein Receptor 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat LOX-1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat LOX-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat LOX-1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat LOX-1. You can calculate the concentration of Rat LOX-1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-102
96-109
95-107
Average (%)
95
103
101
1:4
Range (%)
102-118
91-106
96-111
Average (%)
110
97
102
1:8
Range (%)
99-109
88-104
102-120
Average (%)
104
95
110
1:16
Range (%)
99-115
94-111
87-101
Average (%)
108
101
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
97-110
102
EDTA plasma (n=5)
90-102
96
Cell culture media (n=5)
88-101
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
92.38
308.35
729.63
83.87
321.53
683.58
Standard deviation
11.2
22.05
56.77
7.8
33.92
59.81
C V (%)
12.12
7.15
7.78
9.3
10.55
8.75
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat LOX-1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat LOX-1 in samples. No significant cross-reactivity or interference between Rat LOX-1 and analogues was observed.
